Open AccessConstruction of a shuttle vector for use in Francisella tularensis
Author(s) -
Norqvist Anders,
Kuoppa Kerstin,
Sandström Gunnar
Publication year - 1996
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.1996.tb00248.x
Subject(s) - francisella tularensis , shuttle vector , plasmid , biology , vector (molecular biology) , tularemia , microbiology and biotechnology , virulence , tetracycline , transformation (genetics) , virology , escherichia coli , genetics , gene , antibiotics , recombinant dna
The characterisation of virulence factors of Francisella tularensis has been hampered by the lack of genetic system for the bacterium. In this study, a shuttle vector was constructed that can replicate autonomously in F. tularensis and Escherichia coli . To obtain this vector, the p15A replication origin of E. coli plasmid pACYC184 was introduced into a plasmid derivative of plasmid pFNL200, a plasmid which only can replicate in F. tularensis . The resulting shuttle vector, designated pKK202, harboured resistance genes for chloramphenicol and tetracycline. This vector might be used as a basis for the studies of virulence factors of F. tularensis .