Open AccessOuter membranes of a lipopolysaccharide‐protein complex (LPS‐17 kDa protein) as chemical tularemia vaccines
Author(s) -
Khlebnikov Valentin S.,
Golovliov Igor R.,
Kulevatsky Dmitrij P.,
Tokhtamysheva Natalia V.,
Averin Sergej F.,
Zhemchugov Vladislav E.,
Pchelintsev Sergej Yu.,
Afanasiev Stanislav S.,
Shcherbakov Grigorij Ya.
Publication year - 1996
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.1996.tb00241.x
Subject(s) - tularemia , francisella tularensis , biovar , microbiology and biotechnology , virulence , lipopolysaccharide , bacterial outer membrane , biology , strain (injury) , attenuated vaccine , virology , francisella , bacteria , escherichia coli , immunology , gene , biochemistry , genetics , anatomy
Immunisation with outer membranes of Francisella tularensis induced an efficient protection in guinea pigs against challenge with the virulent strains 503 or 144/713 (type B biovar holarctica ), both clinical isolates, and prevented the development of typical signs of infection in hamadryads (baboons), challenged with the virulent strain Schu (type A, biovar tularensis ) of F. tularensis . Immunisation with a lipopolysaccharide protein complex isolated from the outer membranes afforded protection in CBA mice against challenge with strain 503. Another LPS‐protein complex obtained by the simple mixture of LPS preparations from strain 503 and a 17‐kDa membrane protein from the avirulent R‐variant of the vaccine strain 15 also demonstrated protective properties against experimental tularemia in mice.