Open AccessStructural and immunochemical studies on the lipopolysaccharide of the ‘T‐antigen’‐containing mutant Proteus mirabilis R14/1959
Author(s) -
Bartodziejska Beata,
RadziejewskaLebrecht Joanna,
Lipinska Maria,
Knirel Yuriy A.,
Koov Leonid O.,
Chernyak Anatoly Y.,
Mayer Hubert,
Rozalski Antoni
Publication year - 1996
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.1996.tb00224.x
Subject(s) - proteus mirabilis , antigen , lipopolysaccharide , proteus , biology , antiserum , microbiology and biotechnology , epitope , polyclonal antibodies , strain (injury) , mutant , heterologous , polysaccharide , biochemistry , escherichia coli , genetics , immunology , gene , anatomy
In DOC‐PAGE, lipopolysaccharide (LPS) of Proteus mirabilis R14/1959 (Rb‐type) mutant showed a ladder‐like migration pattern indicating the presence of a high molecular weight polysaccharide chain. The isolated polysaccharide, called T‐antigen because of similarity with the T1 chain of Salmonella friedenau LPS, contained d ‐glucose, d ‐galacturonic acid ( d ‐GalA), and d ‐GlcNAc in molar ratios 2:1:1 and was structurally different from the O‐antigen of the parental S‐strain P. mirabilis S1959 but identical to the O‐antigen of another S‐strain Proteus penneri 42. The importance of a d ‐GalA( l ‐Lys)‐containing epitope, most likely present in the core region of LPS, and of GalA present in the T‐antigen chain in manifesting the serological specificity of P. mirabilis R14/1959 were revealed using rabbit polyclonal homologous and heterologous R‐ and O‐specific antisera and the appropriate antigens, including synthetic antigens which represent partial structures of various Proteus LPS.