Open AccessKnown bacterial virulence factors do not explain the variation in urinary cytokine levels in patients with urosepsis
Author(s) -
Prins Jan M.,
Schultsz Constance,
Speelman Peter,
Deventer Sander J.H.
Publication year - 1996
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.1996.tb00147.x
Subject(s) - urinary system , cytokine , biology , virulence , urine , microbiology and biotechnology , bacteria , in vivo , interleukin 6 , immunology , virulence factor , in vitro , enterobacteriaceae , hemolysin , escherichia coli , endocrinology , gene , biochemistry , genetics
We measured urinary endotoxin, IL‐6 and IL‐8 levels in 23 patients with gram‐negative urosepsis. The endotoxin and cytokine levels showed a 100–1000 fold range. No correlation was found between levels of urinary endotoxin, and IL‐6 or IL‐8 levels. In all cases bacterial numbers were ≥ 10 5 CFU ml −1 urine. The endotoxin content of the isolated microorganisms neither correlated with the urinary cytokine levels, nor with IL‐6 and IL‐8 levels obtained in vitro when 10 3 log‐phase CFU of each of the bacteria were incubated with heparinized whole blood of three healthy donors. Neither the haemolysin phenotype of the bacteria, nor the presence of the P‐pili gene was correlated with the cytokine response in vivo or in vitro. Other factors than known bacterial virulence factors apparently contribute to the wide variation in urinary cytokine levels in urinary tract infection.