Open AccessTreatment of HeLa cells with bacterial water extracts inhibits Shigella flexneri invasion
Author(s) -
DiNovo Bonny Breckinridge,
Doan Richard,
Dyer Roy B.,
Baron Samuel,
Herzog Norbert K.,
Niesel David W.
Publication year - 1996
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.1996.tb00066.x
Subject(s) - shigella flexneri , hela , shigella , biology , microbiology and biotechnology , bacteria , enterobacteriaceae , gentamicin protection assay , plasmid , cell , escherichia coli , western blot , biochemistry , salmonella , gene , genetics
Pathogenesis mediated by Shigella flexneri requires invasion of the gastrointestinal epithelium. It has been previously shown that HeLa cells challenged with S. flexneri show alterations in their phosphotyrosine‐containing protein profile. In this report, we demonstrated that bacterial water extracts (WE) abrogated the invasion of HeLa cells by S. flexneri in a dose‐dependent manner. A proteinaceous component of S. flexneri was shown to be responsible for this inhibitory activity. Proteins encoded on the 140‐MDa plasmid were not responsible for the observed inhibition. WE from other Gram‐negative bacteria also inhibited Shigella invasion of HeLa cells. HeLa cells pretreated with WE showed changes in the profile and the intensity of phosphotyrosine‐containing protein bands. These data were consistent with a surface protein component in WE which initiated aberrant host cell signaling at the membrane which may account for the inhibition of bacterial entry.