Open AccessModulation of Pseudomonas aeruginosa adherence to collagen type I and type II by carbohydrates
Author(s) -
Stȩpińska Małgorzata,
Trafny Elżbieta A.
Publication year - 1995
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.1995.tb00191.x
Subject(s) - pseudomonas aeruginosa , galactose , receptor , mannose , microbiology and biotechnology , biochemistry , type i collagen , biology , type iv collagen , bacteria , chemistry , endocrinology , extracellular matrix , laminin , genetics
This study was undertaken to examine if receptor recognizing saccharides may be involved in the adherence of Pseudomonas aeruginosa to collagen type I and type II. We performed an adherence inhibition assay: cells of individual P. aeruginosa isolates attached to immobilized collagen type I or type II in the presence of monosaccharides, which could serve as blockers of bacterial receptors. Bacterial binding to collagen type I molecules was inhibited to the highest degree by sugar composition d ‐galactose/ d ‐mannose/ N ‐acetylneuraminic acid (5:5:1), whereas attachment of P. aeruginosa to collagen type II was inhibited by composition d ‐glucose/ d ‐galactose (1:1). The same strains which were sensitive to inhibition of binding to collagen type II by both collagen types, were also sensitive to blocking by composition d ‐glucose/ d ‐galactose. It suggests that saccharides play a role in adherence of P. aeruginosa to collagen type I and type II, and a common receptor for both types of collagen may be available on the surface of P. aeruginosa cells.