Open AccessA cell‐free Salmonella typhimurium extract induces inhibition of tyrosine phosphorylation in murine splenic T‐lymphocytes
Author(s) -
Matsui Katsuhiko,
Arai Toshihiko
Publication year - 1995
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.1995.tb00174.x
Subject(s) - biology , phosphorylation , tyrosine phosphorylation , tyrosine , cell growth , microbiology and biotechnology , cell , blot , mitogen activated protein kinase , salmonella , biochemistry , bacteria , genetics , gene
In a previous study, we observed that suppression of T‐cell proliferation induced by Salmonella infection is associated with inhibition of tyrosine phosphorylation in T‐cells, and that a cell‐free Salmonella typhimurium LT2 extract (LT2 extract) also suppressed mitogen‐induced T‐cell proliferation. In the present study, therefore, we attempted to clarify whether the T‐cell suppression induced by LT2 extract involved inhibition of tyrosine phosphorylation in T‐cells. Western blotting using anti‐phosphotyrosine antibodies showed that the mitogen‐induced tyrosine phosphorylation of 120‐, 106‐, 94‐, 76‐, 68‐, 57‐ and 36‐kDa proteins in murine splenic T‐cells was inhibited by treatment with LT2 extract. These results suggest that the suppression of T‐cell proliferation induced by LT2 extract is also associated with inhibition of tyrosine phosphorylation in T‐cells.