Open AccessUse of cyclodextrin as an agent to induce excretion of Bordetella pertussis antigens
Author(s) -
Hozbor Daniela,
Rodriguez Maria E.,
Yantorno Osvaldo
Publication year - 1994
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.1994.tb00482.x
Subject(s) - cyclodextrin , bordetella pertussis , bacterial outer membrane , biology , beta cyclodextrins , extracellular , intracellular , antigen , biochemistry , biophysics , hemagglutinin (influenza) , microbiology and biotechnology , bacteria , immunology , genetics , escherichia coli , gene
This paper attempts to provide an explanation for the effect of cyclodextrin on the yield of Bordetella pertussis soluble antigens. It was demonstrated that the addition of cyclodextrin to the synthetic Stainer‐Scholte liquid medium enhances the level of the intracellular form of adenylate cyclase (200 kDa) in the supernate. In addition to this effect, it has been reported that cyclodextrin also enhances the levels of two other extracellular proteins, pertussis toxin and filamentous hemagglutinin. As these antigens are structurally different, it seems that the effect of cyclodextrin is not specific. With the use of different buffer systems of well‐known action on outer membrane stability it was possible to determine a relationship between the presence of cyclodextrin, destabilisation of the outer membrane and the release of proteins. It was determined that the cyclodextrin did not modify the fluidity of B. pertussis cells but produced a change of outer membrane permeability.