Open AccessInteraction between granulocytes and antibodies in the enhancement of lung defenses against Staphylococcus aureus after intranasal immunization of mice with live‐attenuated bacteria
Author(s) -
García Verónica E.,
Iglesias Mercedes F.,
Cristina Cerquetti M.,
Gómez Marisa I.,
Sordelli Daniel O.
Publication year - 1994
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.1994.tb00474.x
Subject(s) - staphylococcus aureus , microbiology and biotechnology , nasal administration , pseudomonas aeruginosa , immunization , lipopolysaccharide , immunology , antibody , biology , staphylococcal infections , bacteria , genetics
Immunization with live‐attenuated Staphylococcus aureus induced measurable levels of specific IgG and IgA in the lungs, but the pulmonary clearance of S. aureus in immunized mice did not differ from that of control mice. Aerosol exposure of mice to Pseudomonas aeruginosa induced a significant recruitment of polymorphonuclear leukocytes (PMNL) to the lungs in both immunized and control mice, whereas S. aureus challenge did not. However, challenge with a mixture of P. aeruginosa‐S. aureus or exposure to an aerosol of Escherichia coli lipopolysaccharide (LPS) before S. aureus challenge induced PMNL migration and a significant enhancement of pulmonary clearance of S. aureus in immunized mice. The presence of both antibodies and PMNL was required for enhancement of S. aureus pulmonary clearance.