Open AccessLipoarabinomannan from Mycobacterium tuberculosis modulates the generation of reactive nitrogen intermediates by gamma interferon‐activated macrophages
Author(s) -
Anthony Lawrence S.D.,
Chatterjee Delphi,
Brennan Patrick J.,
Nano Francis E.
Publication year - 1994
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.1994.tb00456.x
Subject(s) - lipoarabinomannan , interferon gamma , mycobacterium tuberculosis , microbiology and biotechnology , biology , tuberculosis , interferon γ , mycobacterium , reactive nitrogen species , virology , immunology , bacteria , reactive oxygen species , cytokine , medicine , biochemistry , pathology , genetics
Lipoarabinomannan derived from the virulent Erdman strain and a rapidly growing, laboratory‐attenuated strain of Mycobacterium tuberculosis were evaluated for their ability to modulate the production of nitric oxide (NO) by macrophages activated with IFN‐γ or IFN‐γ and LPS. It was observed that in macrophages pretreated with 100 μg ml −1 LAM, the NO induced by IFN‐γ alone was augmented while the NO induced by IFN‐γ and LPS was reduced. LAM was also shown to synergize with IFN‐γ in the induction of NO, with AraLAM from the attenuated strain exhibiting greater potency than ManLAM from the Erdman strain. Despite the modulation of NO production, LAM did not affect the IFN‐γ‐induced macrophage growth inhibition of Francisella tularensis LVS, an organism whose growth inhibition in activated macrophages is dependent upon NO.