Open AccessLeishmania donovani flagellum‐specific epitopes mediating host‐parasite interactions
Author(s) -
Singla Neena,
Vinayak V.K.
Publication year - 1994
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.1994.tb00440.x
Subject(s) - epitope , leishmania donovani , biology , monoclonal antibody , isotype , visceral leishmaniasis , microbiology and biotechnology , flagellum , leishmania , parasite hosting , antibody , virology , clone (java method) , leishmaniasis , immunology , bacteria , biochemistry , genetics , gene , world wide web , computer science
Monoclonal antibodies were developed against flagellar components of promastigotes of Leishmania donovani . The monoclonal antibody produced by clone A 11 (mAb A 11 ) recognised epitopes in the polypeptides with molecular weights of 86, 66 and weakly 53 kDa. These epitopes were found to be distributed along the flagellum and at the anterior end of promastigotes. The mAb A 11 of IgG 1 isotype strongly agglutinated the promastigotes of L. donovani . The prior treatment of promastigotes of L. donovani with mAb A 11 resulted in a significant ( P < 0.001) reduction in the attachment of promastigotes to cultured mouse peritoneal macrophages of line J774G8. The affinity‐purified epitopes identified by mAb A 11 were recognised by human sera of cases of visceral leishmaniasis. The present study suggest that flagellar‐specific epitopes mediate host‐parasite interactions and, therefore, the role of these epitopes in the disease process is speculated.