Open AccessDiffering signal requirements for the activation of macrophages from C3H/HeJ and C3H/OuJ mice
Author(s) -
AbuLawi Khaled I.,
Sultzer Barnet M.
Publication year - 1994
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.1994.tb00008.x
Subject(s) - ionomycin , protein kinase c , lipopolysaccharide , prostaglandin e2 , biology , activator (genetics) , lymphokine , microbiology and biotechnology , immunology , endocrinology , signal transduction , stimulation , biochemistry , antigen , gene
Endotoxin‐associated protein (EP) from Salmonella typhi stimulated the release of prostaglandin E2 (PGE2), interleukin‐1 (IL‐1), and interferon (IFN) activity in macrophages from the lipopolysaccharide (LPS) responder C3H/OuJ mouse strain. However, only PGE2 and IL‐1 were stimulated by EP in macrophages from the LPS nonresponder C3H/HeJ mouse strain. LPS stimulated the release of PGE2, IL‐1 and IFN activity in C3H/OuJ macrophages, but not in C3H/HeJ macrophages. The protein kinase C (PKC) activator phorbol myristic acid (PMA) stimulated PGE2 production in both strains but not IL‐1 production, suggesting that signalling pathways other than PKC may be involved in IL‐1 production. The calcium ionophore ionomycin stimulated PGE2 production in C3H/OuJ but not C3H/HeJ macrophages, suggesting a defective calcium‐related pathway in the C3H/HeJ macrophages as compared to the C3H/OuJ cells.