Open AccessPeptidoglycan and teichoic acid from Staphylococcus epidermidis stimulate human monocytes to release tumour necrosis factor‐α, interleukin‐1β and interleukin‐6
Author(s) -
Mattsson Eva,
Verhage Liesbeth,
Rollof J.,
Fleer A.,
Verhoef J.,
Dijk H.
Publication year - 1993
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.1993.tb00409.x
Subject(s) - teichoic acid , peptidoglycan , microbiology and biotechnology , staphylococcus epidermidis , lipoteichoic acid , biology , lipopolysaccharide , tumor necrosis factor alpha , interleukin , staphylococcus aureus , cytokine , immunology , biochemistry , cell wall , bacteria , genetics
Cytokines play a major role in the pathophysiology of septic shock. In this study, human periferal blood monocytes were stimulated with peptidoglycan and teichoic acid, purified from a strain of Staphylococcus epidermidis . Polymyxin B (PM‐B) was added to avoid the effects of possible contamination with endotoxin. Tumour necrosis factor‐α (TNF), interleukin‐1β (IL‐1), and interleukin‐6 (IL‐6) in the supernates were measured by enzyme‐linked immunosorbent assays. Peptidoglycan and teichoic acid induced TNF, IL‐1, and IL‐6 in a concentration‐dependent manner. Teichoic acid was a weaker inducer than peptidoglycan, especially for IL‐1. Lipopolysaccharide from an E. coli strain was used as a control, being 100–1000 times more potent than peptidoglycan and teichoic acid.