Open AccessT and B cell responses to cytomegalovirus antigens in healthy blood donors and bone marrow transplant recipients
Author(s) -
Lolli Francesco,
Sundqvist ViviAnne,
Castagna Antonella,
Ljungman Per,
Linde Annika,
Andersson Gudrun,
Olsson Tomas,
Wahren Britta
Publication year - 1993
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.1993.tb00381.x
Subject(s) - antigen , immunology , cytomegalovirus , betaherpesvirinae , antibody , bone marrow , biology , herpesviridae , virology , t cell , immune system , virus , viral disease
We measured the production of interferon‐gamma (IFN‐γ) from single T cells and the T cell proliferative response to different cytomegalovirus (CMV) antigens in healthy blood donors and bone marrow transplant recipients. The antigens consisted of a CMV nuclear antigen (CMV na) containing the pp65‐kDa matrix protein and the immediate early antigens but lacking CMV glycoproteins, and an antigen comprising native CMV glycoproteins (CMV gp). We also measured the IgG antibodies to CMV na and CMV gp. The T cells reacted to CMV na in CMV seropositive blood donors both with the production of IFN‐γ and with proliferation, while bone marrow transplant recipients had a deficient T cell response. After stimulation with CMV gp, no T cell response could be observed in CMV seropositive subjects. IgG antibodies to CMV na coexisted in plasma with similar levels of antibodies to CMV gp.