Open AccessUse of the insertion element IS6110 for DNA fingerprinting of Mycobacterium tuberculosis isolates presenting various profiles of drug susceptibility
Author(s) -
Thierry D.,
MatsiotaBernard P.,
Pitsouni E.,
Costopoulos C.,
Guesdon J.L.
Publication year - 1993
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.1993.tb00341.x
Subject(s) - mycobacterium tuberculosis , restriction fragment length polymorphism , tuberculosis , insertion sequence , biology , dna profiling , molecular epidemiology , genetics , outbreak , dna transposable elements , virology , microbiology and biotechnology , dna , genotype , genome , medicine , transposable element , pathology , gene
IS 6100 is an insertion sequence of the IS3 family and it is present in multiple copies in the chromosome of Mycobacterium tuberculosis . Four to 15 copies are present in various strains of M. tuberculosis . In this study, the value of IS 6110 as an epidemiological marker of tuberculosis was examined. Unrelated clinical strains from Greek patients presented, in restriction fragment length polymorphism analysis, a high degree of polymorphism, whereas patterns of related clinical strains from familial outbreaks were identical. Since RFLP analysis with acetylaminofluorene labeled IS 6110 as the probe gave satisfactory results, it is suggested that this non‐radioactive probe can be used in hospitals and health centres for the epidemiological survey of M. tuberculosis infections.