Open AccessNon‐radioactively labelled DNA probes for the detection of periodontopathogenic Prevotella and Porphyromonas species
Author(s) -
Conrads G.,
Brauner A.
Publication year - 1993
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.1993.tb00313.x
Subject(s) - prevotella intermedia , porphyromonas gingivalis , dot blot , biology , periodontitis , bacteria , microbiology and biotechnology , 16s ribosomal rna , treponema denticola , digoxigenin , hybridization probe , dna , detection limit , in situ hybridization , chromatography , chemistry , medicine , dentistry , genetics , gene , gene expression
Dioxigenin‐labelled synthetic DNA probes directed against the 16S rRNA were used for the direct detection of the periodontopathogenic bacteria Prevotella intermedia and Porphyromonas gingivalis in subgingival plaque by applying a DNA‐RNA dot‐blot hybridization procedure. The test was evaluated with 134 plaque samples from 26 patients with adult periodontitis or rapidly progressive periodontitis. The lower limit of detection was 10 4 –10 5 bacteria/specimen. A semiquantitative assessment of the two species in each sample and in the corresponding periodontal site was achieved by this technique. It is possible to examine 80–90 samples within two days with low material costs.