Open AccessInhibition of interleukin‐6 release and T‐cell proliferation by synthetic mirror pseudo cord factor analogues in human peripheral blood mononuclear cells
Author(s) -
Flad HansDieter,
Baer Hans H.,
Feist Werner,
Ulmer Artur J.
Publication year - 1993
Publication title -
fems immunology & medical microbiology
Language(s) - English
Resource type - Journals
eISSN - 1574-695X
pISSN - 0928-8244
DOI - 10.1111/j.1574-695x.1993.tb00303.x
Subject(s) - phytohaemagglutinin , peripheral blood mononuclear cell , biology , antigen , cytokine , interleukin 2 , cord blood , cell growth , microbiology and biotechnology , lymphocyte , immunology , in vitro , biochemistry
The effects of synthetic alkyl ((alkyl 6‐deoxy‐a‐ d ‐ gluco ‐heptopyranosyluronate) 6‐deoxy‐a‐ D ‐gluco‐heptopyranoside) uronates, a novel type of mirror pseudo cord factor, on the in vitro modulation of interleukin‐6 production and T‐cell proliferation in human peripheral blood mononuclear cells, were investigated. Synthetic mirror pseudo cord factors with alkyl chains ranging from C 16 to C 18 have very weak interleukin‐6‐inducing capacities and lack mitogenic activities for T‐cell proliferation. However, they could inhibit IL‐6 release induced by sonicated Bacillus Calmette‐Guérin (S‐BCG), bacterial endotoxin, and phytohaemagglutinin in a dose‐dependent manner. Inhibition was observed not only with mononuclear cells but also with purified monocytes. Furthermore, these synthetic compounds could suppress T‐lymphocyte proliferation stimulated by sonicated Mycobacterium tuberculosis H37Rv (S‐H37Rv) antigens, S‐BCG antigens, as well as by recombinant 65 kDa mycobacterial heat‐shock protein. In contrast, these compounds failed to inhibit the phytohaemagglutinin‐induced T‐cell proliferation. We conclude that the inhibition of cytokine release and T‐cell proliferation by synthetic mirror pseudo cord factors was due to direct blocking of the function and/or activity of monocytes or antigen‐presenting cells.