Open AccessCo‐occurrence of microcystin and microginin congeners in B razilian strains of M icrocystis sp.
Author(s) -
Carneiro Ronaldo Leal,
Dörr Felipe Augusto,
Dörr Fabiane,
Bortoli Stella,
Delherbe Nathalie,
Vásquez Mónica,
Pinto Ernani
Publication year - 2012
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.2012.01439.x
Subject(s) - microcystis , microcystin , biology , cyanobacteria , biosynthesis , botany , cyanotoxin , microbiology and biotechnology , bacteria , biochemistry , gene , genetics
Species of M icrocystis are the most common bloom‐forming cyanobacteria in several countries. Despite extensive studies regarding the production of bioactive cyanopeptides in this genus, there are limited data on isolated strains from B razil. Three M icrocystis sp. strains were isolated from the S alto G rande R eservoir ( LTPNA 01, 08 and 09) and investigated for the presence of mcy genes, microcystins and other cyanopeptides. Microcystin and microginin production was confirmed in two isolates using high‐resolution tandem mass spectrometry after electrospray ionization ( ESI ‐ Q ‐ TOF ), and the structures of two new microginin congeners were proposed ( MG 756 A hda‐ V al‐ L eu‐ H ty‐ T yr and MG 770 M e A hda‐ V al‐ L eu‐ H ty‐ T yr). The biosynthesis profile of the identified cyanopeptides was evaluated at different growth phases via a newly developed HPLC ‐ UV method. Results demonstrated no substantial differences in the production of microcystins and microginins after data normalization to cell quota, suggesting a constitutive biosynthesis. This study represents the first confirmed co‐production of microginins and microcystins in B razilian strains of M icrocystis sp. and highlights the potential of B razilian cyanobacteria as a source of natural compounds with pharmaceutical interest.