Open AccessMolecular and culture‐dependent analyses revealed similarities in the endophytic bacterial community composition of leaves from three rice ( O ryza sativa ) varieties
Author(s) -
Ferrando Lucía,
Mañay Jimena Fernández,
Scavino Ana Fernández
Publication year - 2012
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.2012.01339.x
Subject(s) - biology , oryza sativa , restriction fragment length polymorphism , terminal restriction fragment length polymorphism , pseudomonas , botany , 16s ribosomal rna , crop , pantoea , library , bacteria , oryza , genotype , agronomy , gene , genetics
The endophytic bacterial communities of the three most important rice varieties cultivated in U ruguay were compared by a multiphasic approach. Leaves of mature plants grown in field experiments for two consecutive crop seasons were studied. No significant differences were found in the heterotrophic bacterial density for the three varieties. P antoea ananatis and P seudomonas syringae constituted 51% of the total of the isolates. These species were always present regardless of the variety or the season. Molecular analysis based on the 16 S rRNA gene was performed by terminal restriction fragment length polymorphism ( T ‐ RFLP ) and cloning. T ‐ RFLP analysis revealed that bacterial communities grouped according to the variety, although the three varieties presented communities that showed 74% or higher similarities. B revundimonas , the dominant genus in the clone library (18% of the clones), which might be present in all varieties according to T ‐ RFLP profiles, was not recovered by cultivation. Conversely, bacteria from the genus P seudomonas were not detected in the clone library. These results indicate that communities established in leaves of physiologically different rice varieties were highly similar and composed by a reduced group of strongly associated and persistent bacteria that were partially recovered by cultivation.