Open AccessIncrease in antioxidant gene transcripts, stress tolerance and biocontrol efficacy of C andida oleophila following sublethal oxidative stress exposure
Author(s) -
Liu Jia,
Wisniewski Michael,
Droby Samir,
Norelli John,
Hershkovitz Vera,
Tian Shiping,
Farrell Robert
Publication year - 2012
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.2012.01324.x
Subject(s) - biology , oxidative stress , suppression subtractive hybridization , penicillium expansum , yeast , reactive oxygen species , microbiology and biotechnology , botrytis cinerea , gene , gene expression , botany , biochemistry , postharvest , cdna library
A pretreatment of the yeast, C andida oleophila , with 5 mM H 2 O 2 for 30 min (sublethal) increased yeast tolerance to subsequent lethal levels of oxidative stress (50 mM H 2 O 2 ), high temperature (40 °C), and low p H (p H 4). Compared with non‐stress‐adapted yeast cells, stress‐adapted cells exhibited better control of apple fruit infections by P enicillium expansum and B otrytis cinerea and had initially higher growth rates in apple wounds. Suppression subtractive hybridization analysis was used to identify genes expressed in yeast in response to sublethal oxidative stress. Transcript levels were confirmed using semiquantitative reverse transcription‐ PCR . Seven antioxidant genes were upregulated. The elevated expression of these genes was associated with less accumulation of reactive oxygen species and a lower level of protein and lipid oxidation under subsequent stresses. These data support the premise that induction of abiotic stress tolerance in biocontrol yeast can improve biocontrol efficacy by upregulation of genes involved in the amelioration of oxidative stress.