Open AccessPhylogenetic diversity, composition and distribution of bacterioplankton community in the D ongjiang R iver, C hina
Author(s) -
Liu Zhenghui,
Huang Shaobin,
Sun Guoping,
Xu Zhencheng,
Xu Meiying
Publication year - 2012
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.2011.01268.x
Subject(s) - gammaproteobacteria , betaproteobacteria , biology , alphaproteobacteria , actinobacteria , bacterioplankton , deltaproteobacteria , temperature gradient gel electrophoresis , bacteroidetes , ecology , proteobacteria , acidobacteria , planctomycetes , phylogenetic diversity , canonical correspondence analysis , library , 16s ribosomal rna , phylogenetic tree , abundance (ecology) , bacteria , phytoplankton , nutrient , biochemistry , genetics , gene
Bacterioplankton community compositions in the D ongjiang R iver were characterized using denaturing gradient gel electrophoresis ( DGGE ) and 16 S r RNA gene clone library construction. Water samples in nine different sites were taken along the mainstem and three tributaries. In total, 24 bands from DGGE gels and 406 clones from the libraries were selected and sequenced, subsequently analyzed for the bacterial diversity and composition of those microbial communities. Bacterial 16 S r RNA gene sequences from freshwater bacteria exhibited board phylogenetic diversity, including sequences representing the A lphaproteobacteria , B etaproteobacteria , G ammaproteobacteria , A ctinobacteria , B acteriodetes , V errucomicrobia, and candidate division TM 7. Members of B etaproteobacteria group were the most dominant in all sampling sites, followed by G ammaproteobacteria, A lphaproteobacteria, and A ctinobacteria . DGGE profiles and the ∫‐ libshuff analysis revealed similar patterns of bacterial diversity among most sampling sites, while spatial distribution variances existed in all sites along the river basin. Statistical analysis showed that bacterial species distribution strongly correlated with environmental variables, such as nitrate and ammonia, suggesting that nitrogen nutrients may shape the microbial community structure and composition in the D ongjiang R iver. This study had important implications for the comparison with other rivers elsewhere and contributed to the growing data set on the factors that structure bacterial communities in freshwater ecosystems.