Open AccessDecreased colonization of fecal C lostridium coccoides / E ubacterium rectale species from ulcerative colitis patients in an in vitro dynamic gut model with mucin environment
Author(s) -
Vermeiren Joan,
den Abbeele Pieter,
Laukens Debby,
Vigsnæs Louise Kristine,
Vos Martine,
Boon Nico,
Wiele Tom
Publication year - 2012
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.2011.01252.x
Subject(s) - faecalibacterium prausnitzii , biology , eubacterium , butyrate , roseburia , microbiology and biotechnology , mucin , feces , gut flora , mucus , ulcerative colitis , short chain fatty acid , bacteroides , food science , bacteria , immunology , biochemistry , medicine , fermentation , ecology , genetics , disease
The mucus layer in the colon, acting as a barrier to prevent invasion of pathogens, is thinner and discontinuous in patients with ulcerative colitis ( UC ). A recent developed in vitro dynamic gut model, the M ‐ SHIME , was used to compare long‐term colonization of the mucin layer by the microbiota from six healthy volunteers ( HV ) and six UC patients and thus distinguish the mucin adhered from the luminal microbiota. Although under the same nutritional conditions, short‐chain fatty acid production by the luminal communities from UC patients showed a tendency toward a lower butyrate production. A more in‐depth community analysis of those microbial groups known to produce butyrate revealed that the diversity of the C lostridium coccoides / E ubacterium rectale and C lostridium leptum group, and counts of F aecalibacterium prausnitzii were lower in the luminal fractions of the UC samples. Counts of R oseburia spp. were lower in the mucosal fractions of the UC samples. q PCR analysis for butyryl‐ C o A :acetate C o A transferase, responsible for butyrate production, displayed a lower abundance in both the luminal and mucosal fractions of the UC samples. The M ‐ SHIME model revealed depletion in butyrate producing microbial communities not restricted to the luminal but also in the mucosal samples from UC patients compared to HV .