Open AccessGenetic diversity of M imosa pudica rhizobial symbionts in soils of F rench G uiana: investigating the origin and diversity of B urkholderia phymatum and other beta‐rhizobia
Author(s) -
Mishra Ravi P.N.,
Tisseyre Pierre,
Melkonian Rémy,
Chaintreuil Clémence,
Miché Lucie,
Klonowska Agnieszka,
Gonzalez Sophie,
Bena Gilles,
Laguerre Gisèle,
Moulin Lionel
Publication year - 2012
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.2011.01235.x
Subject(s) - biology , rhizobia , botany , burkholderia , 16s ribosomal rna , nitrogen fixation , genetic diversity , symbiosis , rhizobium , gene , genetics , bacteria , population , demography , sociology
The genetic diversity of 221 M imosa pudica bacterial symbionts trapped from eight soils from diverse environments in F rench G uiana was assessed by 16 S rRNA PCR ‐ RFLP , REP ‐ PCR fingerprints, as well as by phylogenies of their 16 S rRNA and recA housekeeping genes, and by their nifH , nodA and nodC symbiotic genes. Interestingly, we found a large diversity of beta‐rhizobia, with B urkholderia phymatum and B urkholderia tuberum being the most frequent and diverse symbiotic species. Other species were also found, such as B urkholderia mimosarum , an unnamed B urkholderia species and, for the first time in S outh A merica, C upriavidus taiwanensis . The sampling site had a strong influence on the diversity of the symbionts sampled, and the specific distributions of symbiotic populations between the soils were related to soil composition in some cases. Some alpha‐rhizobial strains taxonomically close to R hizobium endophyticum were also trapped in one soil, and these carried two copies of the nodA gene, a feature not previously reported. Phylogenies of nodA , nodC and nifH genes showed a monophyly of symbiotic genes for beta‐rhizobia isolated from M imosa spp., indicative of a long history of interaction between beta‐rhizobia and M imosa species. Based on their symbiotic gene phylogenies and legume hosts, B . tuberum was shown to contain two large biovars: one specific to the mimosoid genus M imosa and one to S outh A frican papilionoid legumes.