Open AccessImpact of rhizosphere factors on cyclic lipopeptide signature from the plant beneficial strain B acillus amyloliquefaciens S 499
Author(s) -
Nihorimbere Venant,
Cawoy Hélène,
Seyer Alexandre,
Brunelle Alain,
Thonart Philippe,
Ongena Marc
Publication year - 2012
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.2011.01208.x
Subject(s) - surfactin , bacillus amyloliquefaciens , rhizosphere , lipopeptide , biology , context (archaeology) , biofilm , microbiology and biotechnology , bacillus subtilis , bacteria , strain (injury) , bacillus (shape) , biological pest control , botany , biochemistry , food science , fermentation , paleontology , genetics , anatomy
Cyclic lipopeptides ( cLPs ) of the surfactin, iturin and fengycin families synthesized by plant‐associated B acilli represent an important class of antibiotics as they may be tightly involved in the protective effect of selected strains against phytopathogens. However, their production by B acillus cells developing on roots under rhizosphere conditions is still poorly understood. In this work, we combined electrospray and imaging mass spectrometry‐based approaches to determine the detailed pattern of surfactins, iturins and fengycins produced in planta by B acillus amyloliquefaciens S 499. Very different production rates were observed for the three cLPs families. Whereas surfactin accumulated in significant amounts, much lower quantities of iturins and fengycins were detected in the environment of colonized roots in comparison with laboratory medium. In addition, the surfactin pattern produced by strain S 499 evolving on roots is enriched in homologues with long fatty acid chains ( C 15) compared with the chains typically secreted under in vitro conditions. Additional experiments revealed that lipopeptide production by root‐associated S 499 cells is qualitatively and quantitatively dictated by the specific nutritional context of the rhizosphere (exudates enriched in organic acids, oxygen limitation) but also by the formation of biofilm‐related structures around root hairs. As surfactins, iturins and fengycins retain specific functions and bioactivities, the biological relevance of their differential production observed in planta is discussed in the context of biocontrol of plant diseases.