Open AccessThe phylogenetic distribution and ecological role of carbon monoxide oxidation in the genus B urkholderia
Author(s) -
Weber Carolyn F.,
King Gary M.
Publication year - 2012
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.2011.01206.x
Subject(s) - biology , burkholderia , rhizosphere , microbiology and biotechnology , bacteria , burkholderia cepacia complex , phylogenetic tree , botany , biochemistry , gene , genetics
B urkholderia is a physiologically and ecologically diverse genus that occurs commonly in assemblages of soil and rhizosphere bacteria. Although B urkholderia is known for its heterotrophic versatility, we demonstrate that 14 distinct environmental isolates oxidized carbon monoxide ( CO ) and possessed the gene encoding the catalytic subunit of form I CO dehydrogenase ( coxL ). DNA from a B urkholderia isolate obtained from a passalid beetle also contained coxL as do the genomic sequences of species H 160 and C h1‐1. Isolates were able to consume CO at concentrations ranging from 100 ppm (vol/vol) to sub‐ambient (< 60 ppb (vol/vol)). High concentrations of pyruvate inhibited CO uptake (> 2.5 mM ), but mixotrophic consumption of CO and pyruvate occurred when initial pyruvate concentrations were lower ( c . 400 μ M ). With the exception of an isolate most closely related to B urkholderia cepacia , all CO ‐oxidizing isolates examined were members of a nonpathogenic clade and were most closely related to B urkholderia species, B. caledonica , B. fungorum , B. oxiphila , B. mimosarum , B. nodosa , B. sacchari , B. bryophila , B. ferrariae , B. ginsengesoli , and B. unamae . However, none of these type strains oxidized CO or contained coxL based on results from PCR analyses. Collectively, these results demonstrate that the presence of CO oxidation within members of the B urkholderia genus is variable but it is most commonly found among rhizosphere inhabitants that are not closely related to B . cepacia .