Open AccessPostnatal development of the rabbit caecal microbiota composition and activity
Author(s) -
Combes Sylvie,
Michelland Rory Julien,
Monteils Valérie,
Cauquil Laurent,
Soulié Vincent,
Tran Ngoc Uyen,
Gidenne Thierry,
FortunLamothe Laurence
Publication year - 2011
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.2011.01148.x
Subject(s) - biology , firmicutes , prevotella , caecum , bacteroidetes , bacteroides , population , microbiology and biotechnology , bacteria , 16s ribosomal rna , zoology , genetics , medicine , demography , sociology
This study describes the development of the rabbit caecum microbiota and its metabolic activities from the neonatal (day 2) until the subadult period (day 70). The caecal microbiota was analysed using 16S rRNA gene approaches coupled with capillary electrophoresis single‐stranded conformation polymorphism (CE‐SSCP) and qPCR. At day 2, rabbits harboured population levels up to 8.4, 7.2 and 7.4 log 10 copy number g −1 full caecum of the total bacteria, Bacteroides – Prevotella and Firmicutes groups, respectively. These populations reached their maximum levels from day 14 for Firmicutes groups (10.8 log 10 copy number g −1 caecal content) and day 21 (11.4 and 10.7 log 10 copy number g −1 caecal content of the total bacteria and the Bacteroides – Prevotella group, respectively). The archaeal population could be detected only from day 7 onwards (5.5 log 10 copy number g −1 full caecum) and reached its maximum level at day 35 (7.4 log 10 copy number g −1 caecal content). Similarity analysis, diversity calculation and quantitative evaluation of the stability of bacterial community CE‐SSCP profiles provided some evidence that the caecal microbiota develops progressively from a simple and unstable community after birth into a complex and climax community in subadult rabbits. Meanwhile, the microbial activity evolved with the progressive decrease of the propionate/butyrate ratio towards a rabbit‐specific value <1.