Open AccessEstablishment of intestinal microbiota with focus on yeasts of unweaned and weaned piglets kept under different farm conditions
Author(s) -
Urubschurov Vladimir,
Janczyk Pawel,
Souffrant WolfgangBernhard,
Freyer Gertraude,
Zeyner Annette
Publication year - 2011
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.2011.01129.x
Subject(s) - biology , temperature gradient gel electrophoresis , weaning , feces , yeast , microbiology and biotechnology , bacteria , lactobacillus , isolation (microbiology) , food science , 16s ribosomal rna , zoology , genetics
This study aimed to characterize the intestinal yeasts in weaning piglets and to establish their possible relationships with main bacterial groups. German Landrace piglets were weaned (WP, n =32) at 28 days of age or kept with the dams until day 39 without creep feed (UP, n =32). The experiment was performed at an experimental and a commercial farm (CF). Faeces were collected from the piglets, sows and pen floors on days 28, 33 and 39 for isolation of DNA and cultivation for enumeration of yeasts, enterobacteria, enterococci and lactobacilli. Fragments of the D1 domain of 26S rRNA gene were amplified and separated by denaturing gradient gel electrophoresis (DGGE). No yeasts could be cultured from water and feed samples. No or only low numbers of yeasts were detected among all UP. In WP at CF, yeasts correlated with lactobacilli ( r =0.456; P =0.009) and enterobacteria ( r =−0.407; P =0.021). Kazachstania slooffiae dominated among the cultured yeasts. It was the only yeast species detected by PCR‐DGGE. Yeasts, especially K. slooffiae , established in the porcine gastrointestinal tract after consumption of grain‐based feed and may interrelate with the intestinal microbiota. The study provides data indicating importance of K. slooffiae for the development of balanced porcine gut microbiota.