Open AccessIntracellular Oceanospirillales bacteria inhabit gills of Acesta bivalves
Author(s) -
Jensen Sigmund,
Duperron Sébastien,
Birkeland NilsKåre,
Hovland Martin
Publication year - 2010
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.2010.00981.x
Subject(s) - biology , gammaproteobacteria , gill , bacteria , 16s ribosomal rna , ribosomal rna , ribosomal dna , microbiology and biotechnology , zoology , phylogenetics , gene , genetics , fishery , fish <actinopterygii>
A novel bacterium was discovered in the gills of the large bivalve Acesta excavata ( Limidae ) from coral reefs on the northeast Atlantic margin near the shelf break of the fishing ground Haltenbanken of Norway, and confirmed present in A. excavata from a rock‐wall in the Trondheimsfjord. Purified gill DNA contained one dominant bacterial rRNA operon as indicated from analysis of broad range bacterial PCR amplicons in denaturant gradient gels, in clone libraries and by direct sequencing. The sequences originated from an unknown member of the order Oceanospirillales and its 16S rRNA gene fell within a clade of strictly marine invertebrate‐associated Gammaproteobacteria . Visual inspection by fluorescent in situ hybridization and transmission electron microscopy indicated a pleomorphic bacterium with no visible cell wall, located in aggregates inside vacuoles scattered within the gill cells cytoplasm. Intracellular Oceanospirillales exist in bathymodiolin mussels (parasites), Osedax worms and whiteflies (symbionts). This bacterium apparently lives in a specific association with the Acesta .