Open AccessImproved PCR primers for the detection and identification of arbuscular mycorrhizal fungi
Author(s) -
Lee Jaikoo,
Lee Sangsun,
Young J. Peter W.
Publication year - 2008
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.2008.00531.x
Subject(s) - biology , glomeromycota , primer (cosmetics) , ascomycota , phylogenetic tree , arbuscular mycorrhizal fungi , botany , polymerase chain reaction , gene , genetics , mycorrhiza , symbiosis , bacteria , horticulture , chemistry , organic chemistry , inoculation
A set of PCR primers that should amplify all subgroups of arbuscular mycorrhizal fungi (AMF, Glomeromycota ), but exclude sequences from other organisms, was designed to facilitate rapid detection and identification directly from field‐grown plant roots. The small subunit rRNA gene was targeted for the new primers (AML1 and AML2) because phylogenetic relationships among the Glomeromycota are well understood for this gene. Sequence comparisons indicate that the new primers should amplify all published AMF sequences except those from Archaeospora trappei . The specificity of the new primers was tested using 23 different AMF spore morphotypes from trap cultures and Miscanthus sinensis , Glycine max and Panax ginseng roots sampled from the field. Non‐AMF DNA of 14 plants, 14 Basidiomycota and 18 Ascomycota was also tested as negative controls. Sequences amplified from roots using the new primers were compared with those obtained using the established NS31 and AM1 primer combination. The new primers have much better specificity and coverage of all known AMF groups.