Open AccessBiogeography of sulfate‐reducing prokaryotes in river floodplains
Author(s) -
Miletto Marzia,
Loy Alexander,
Antheunisse A. Martijn,
Loeb Roos,
Bodelier Paul L.E.,
Laanbroek Hendrikus J.
Publication year - 2008
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.2008.00490.x
Subject(s) - biology , temperature gradient gel electrophoresis , floodplain , sulfate , environmental chemistry , ecology , 16s ribosomal rna , bacteria , genetics , chemistry , organic chemistry
In this study, a large‐scale field survey was conducted to describe the biogeography of sulfate‐reducing prokaryotes (SRPs) in river floodplains. Fingerprints obtained with three methods, i.e. 16S rRNA gene‐based oligonucleotide microarray, dsrB ‐based denaturing gradient gel electrophoresis (DGGE) and polar lipid‐derived fatty acid (PLFA) analyses, were used as a proxy to describe the SRPs community diversity. Each set of profiles was subjected to a combined multivariate/correlation analysis in order to compare SRP community profiles and to highlight the environmental variables influencing the SRPs distribution along environmental gradients. Floodplain soils harbored distinct SRP communities displaying biogeographic patterns. Nearly all profiles from the tidal sites consistently separated from the nontidal sites, independently from the screening method and the multivariate statistics used. The distribution of the microarray/DGGE/PLFA‐based fingerprints in the principal component plots could be correlated to eight soil variables, i.e. soil organic matter, total nitrogen, total phosphorous and total potassium, and extractable ammonium, nitrate, phosphate and sulfate, as well as seven pore water variables, i.e. phosphate, sulfate, sulfide, chloride, sodium, potassium and magnesium ions. Indication of a salinity‐ and plant nutrient‐dependent distribution of SRPs related to Desulfosarcina , Desulfomonile and Desulfobacter was suggested by microarray, DGGE and PLFA analyses.