Open AccessChanges in Escherichia coli outer membrane subproteome under environmental conditions inducing the viable but nonculturable state
Author(s) -
Muela Alicia,
Seco Carolina,
Camafeita Emilio,
Arana Inés,
Orruño Maite,
López Juan Antonio,
Barcina Isabel
Publication year - 2008
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.2008.00453.x
Subject(s) - viable but nonculturable , escherichia coli , proteome , biology , microbiology and biotechnology , bacterial outer membrane , population , enterobacteriaceae , bacteria , biochemistry , genetics , demography , sociology , gene
Changes in the outer membrane subproteome of Escherichia coli along the transition to the viable but nonculturable state (VBNC) were studied. The VBNC state was triggered by exposure of E. coli cells to adverse conditions such as aquatic systems, starvation, suboptimal temperature, visible light irradiation and seawater. The subproteome, obtained according to Molloy et al ., was analysed at the beginning of exposure (inoculum, phase 1), after a variable exposure time (95% of population culturable, phase 2) and when populations were mainly in the VBNC state (95% of cells VBNC, phase 3). Proteome changes were dependent on adverse conditions inducing the transition and were detected mainly in phase 2. The permanence of E. coli cells in seawater under illumination conditions entailed a dramatic rearrangement of the outer membrane subproteome involving 106 new spots, some of which could be identified by peptide fingerprinting. However, proteins exclusive to the VBNC state were not detected.