Open AccessN ‐acyl homoserine lactone‐degrading microbial enrichment cultures isolated from Penaeus vannamei shrimp gut and their probiotic properties in Brachionus plicatilis cultures
Author(s) -
Tinh Nguyen Thi Ngoc,
Asanka Gunasekara R.A.Y.S.,
Boon Nico,
Dierckens Kristof,
Sorgeloos Patrick,
Bossier Peter
Publication year - 2007
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.2007.00378.x
Subject(s) - vibrio harveyi , biology , microbiology and biotechnology , shrimp , quorum sensing , autoinducer , brachionus , probiotic , penaeus , vibrio , vibrionaceae , bacteria , biochemistry , virulence , fishery , ecology , gene , rotifer , genetics
Three bacterial enrichment cultures (ECs) were isolated from the digestive tract of Pacific white shrimp Penaeus vannamei , by growing the shrimp microbial communities in a mixture of N ‐acyl homoserine lactone (AHL) molecules. The ECs, characterized by denaturing gradient gel electrophoresis analysis and subsequent rRNA sequencing, degraded AHL molecules in the degradation assays. Apparently, the resting cells of the ECs also degraded one of the three types of quorum‐sensing signal molecules produced by Vibrio harveyi in vitro [i.e. harveyi autoinducer 1 (HAI‐1)]. The most efficient AHL‐degrading ECs, EC5, was tested in Brachionus experiments. EC5 degraded the V. harveyi HAI‐1 autoinducer in vivo , neutralizing the negative effect of V. harveyi autoinducer 2 (AI‐2) mutant, in which only the HAI‐1‐ and CAI‐1‐mediated components of the quorum‐sensing system are functional on the growth of Brachionus . This suggests that EC5 interferes with HAI‐1‐regulated metabolism in V. harveyi . These AHL‐degrading ECs need to be tested in other aquatic systems for their probiotic properties, preferably in combination with specific AI‐2‐degrading bacteria.