Open AccessLack of correspondence between genetic and phenotypic groups amongst soil‐borne streptomycetes
Author(s) -
Davelos Baines Anita L.,
Xiao Kun,
Kinkel Linda L.
Publication year - 2007
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.2006.00231.x
Subject(s) - biology , 16s ribosomal rna , genetics , gene , phenotype , ribosomal rna , streptomyces , strain (injury) , phenotypic trait , sequence (biology) , bacteria , anatomy
Correspondence between two distinct genetic traits, 16S rRNA gene sequences and repetitive element‐sequence‐based BOX‐PCR DNA fingerprints, and antibiotic inhibition and resistance phenotypes was explored for a spatially explicit sample of Streptomyces from a prairie soil. There was no correspondence between 16S rRNA gene sequence groups and antibiotic phenotypes. However, 16S rRNA gene sequence groups differed significantly in mean inhibition zone sizes. Specific antibiotic phenotypes may reflect local selection pressures, as suggested by the significant differences in mean inhibition zone sizes against specific test isolates by Streptomyces from the same 16S rRNA gene sequence group but from different locations in soil. Significant correlations between antibiotic phenotypes and BOX‐PCR fingerprints were found, but were small ( r =0.19–0.22). Although genetic characterizations alone were not predictive of specific antibiotic phenotypes, 16S rRNA gene sequence analyses may identify isolates that are most or least likely to possess substantial inhibitory potential, providing insight into the broad ecological strategy for individual isolates.