Open Access16S rRNA gene‐based analysis of mucosa‐associated bacterial community and phylogeny in the chicken gastrointestinal tracts: from crops to ceca
Author(s) -
Gong Jianhua,
Si Weiduo,
Forster Robert J.,
Huang Ruilin,
Yu Hai,
Yin Yulong,
Yang Chengbo,
Han Yanming
Publication year - 2007
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.2006.00193.x
Subject(s) - biology , ileum , microbiology and biotechnology , 16s ribosomal rna , ruminococcus , lactobacillus salivarius , jejunum , lactobacillus , gastrointestinal tract , faecalibacterium prausnitzii , microbiome , bacteria , genetics , feces , biochemistry , endocrinology
Mucosa‐associated microbiota from different regions of the gastrointestinal (GI) tract of adult broilers was studied by analysis of 16S rRNA gene sequences. The microbiota mainly comprised Gram‐positive bacteria along the GI tract. Fifty‐one operational taxonomic units (OTUs) (from 98 clones) were detected in the ceca, as compared with 13 OTUs (from 49 clones) in the crops, 11 OTUs (from 51 clones) in the gizzard, 14 OTUs (from 52 clones) in the duodenum, 12 OTUs (from 50 clones) in the jejunum and nine OTUs (from 50 clones) in the ileum. Ceca were dominantly occupied by clostridia‐related sequences (40%) with other abundant sequences being related to Faecalibacterium prausnitzii (14%), Escherichia coli (11%), lactobacilli (7%) and Ruminococcus (6%). Lactobacilli were predominant in the upper GI tract and had the highest diversity in the crop. Both Lactobacillus aviarius and Lactobacillus salivarius were the predominant species among lactobacilli. Candidatus division Arthromitus was also abundant in the jejunum and ileum.