Open AccessMicrobial composition of chlorinated ethene‐degrading cultures dominated by Dehalococcoides
Author(s) -
Duhamel Melanie,
Edwards Elizabeth A.
Publication year - 2006
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.2006.00191.x
Subject(s) - phylotype , biology , methanosaeta , microbiology and biotechnology , euryarchaeota , temperature gradient gel electrophoresis , dehalococcoides , 16s ribosomal rna , enrichment culture , methanomicrobiales , vinyl chloride , gene , bacteria , genetics , archaea , methanosarcina , chemistry , polymer , organic chemistry , copolymer
The community composition of microbial cultures degrading tetrachloroethene (PCE), trichloroethene (TCE), cis‐ 1,2‐dichloroethene (cDCE) and vinyl chloride (VC) to ethene was studied. A combination of PCR‐denaturing gradient gel electrophoresis (PCR‐DGGE) and 16S rRNA gene sequence analysis revealed that all cultures contained Dehalococcoides populations, but that the populations of other organisms varied widely. Based on the sequences of cloned 16S rRNA genes, real‐time PCR methods were developed for several of these phylotypes affiliated with the putative dechlorinators Sulfurospirillum and Geobacter , the putative methanogens Methanomethylovorans , Methanomicrobiales, Methanosaeta and Methanosarcina , the putative acetogens Acetobacterium , Spirochaetes, and Sporomusa , and the putative fermenters Bacteroidetes , Syntrophus , and Syntrophobacter . These novel quantitative PCR methods were then used to estimate relative abundances of each phylotype in several individual cultures maintained on each chlorinated ethene. Dehalococcoides populations were the dominant phylotypes assayed in most KB‐1 cultures, agreeing with the DGGE and cloning results. A Geobacter phylotype was also strongly represented in most PCE and TCE cultures, but not in cDCE or VC cultures, suggesting a possible role for this organism as a PCE‐to‐cDCE dechlorinator. The Sulfurospirillum phylotype was estimated to comprise a minor fraction of 16S rRNA gene copies and did not appear to have an important role in dechlorination.