Open AccessEndobacteria in some ectomycorrhiza of Scots pine ( Pinus sylvestris )
Author(s) -
Izumi Hironari,
Anderson Ian C.,
Alexander Ian J.,
Killham Ken,
Moore Edward R.B.
Publication year - 2006
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.2005.00048.x
Subject(s) - biology , botany , russula , scots pine , ectomycorrhiza , mycorrhiza , symbiosis , pinus <genus> , bacteria , genetics
The diversity of cultivable endobacteria associated with four different ectomycorrhizal morphotypes ( Suillus flavidus , Suillus variegatus , Russula paludosa and Russula sp.) of Scots pine ( Pinus sylvestris ) was analysed by restriction fragment length polymorphism profiling of PCR‐amplified rDNA intergenic spacer regions and by sequence analyses of 16S rRNA genes. Ectomycorrhizal root tip surface‐sterilization methods were developed and assessed for their efficiencies. Bacterial communities from surface‐sterilized ectomycorrhizal root tips were different from those of ectomycorrhizal root tips without surface‐sterilization for all the morphotypes studied. Endobacteria belonging to the genera Pseudomonas , Burkholderia and Bacillus were isolated from more than one ectomycorrhizal morphotype, whereas species of Rahnella , Janthinobacterium and Rhodococcus were only isolated from the single morphotypes of S. variegatus , R. paludosa and Russula sp., respectively. Some of the isolated endobacteria utilized fungal sugars more readily than typical plant sugars in carbon utilization assays.