Open AccessHow elevated pCO 2 modifies total and metabolically active bacterial communities in the rhizosphere of two perennial grasses grown under field conditions
Author(s) -
Jossi Maryline,
Fromin Nathalie,
Tarnawski Sonia,
Kohler Florian,
Gillet François,
Aragno Michel,
Hamelin Jérôme
Publication year - 2006
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.2005.00040.x
Subject(s) - biology , lolium perenne , temperature gradient gel electrophoresis , actinobacteria , rhizosphere , perennial plant , proteobacteria , lolium , botany , 16s ribosomal rna , bacteria , poaceae , genetics
The response of total (DNA‐based analysis) and active (RNA‐based analysis) bacterial communities to a pCO 2 increase under field conditions was assessed using two perennial grasses: the nitrophilic Lolium perenne and the oligonitrophilic Molinia coerulea . PCR‐ and reverse transcriptase‐PCR denaturing gradient gel electrophoresis analysis of 16S rRNA genes generated contrasting profiles. The pCO 2 increase influenced mainly the active and root‐associated component of the bacterial community. Bacterial groups responsive to the pCO 2 increase were identified by sequencing of corresponding denaturing gradient gel electrophoresis bands. About 50% of retrieved sequences were affiliated to Proteobacteria . Our data suggest that Actinobacteria in soil and Myxococcales ( Deltaproteobacteria ) in root are stimulated under elevated pCO 2 .