Open AccessMolecular markers detecting an ectomycorrhizal Suillus collinitus strain on Pinus halepensis roots suggest successful inoculation and persistence in Mediterranean nursery and plantation
Author(s) -
Karkouri Khalid El,
Selosse MarcAndré,
Mousain Daniel
Publication year - 2006
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.2005.00014.x
Subject(s) - biology , inoculation , ectomycorrhiza , ribosomal dna , botany , microbial inoculant , internal transcribed spacer , restriction fragment length polymorphism , population , mycorrhiza , strain (injury) , pinus <genus> , symbiosis , ribosomal rna , horticulture , polymerase chain reaction , phylogenetics , gene , genetics , bacteria , demography , anatomy , sociology
Survival of the ectomycorrhizal fungal strain Suillus collinitus Sc‐32 on Pinus halepensis after inoculation and outplanting was monitored in a Mediterranean plantation. Three molecular fingerprints were developed: RFLP of the internal transcribed spacer ribosomal DNA, intersimple sequence repeat, and a specific sequence‐characterized amplified region marker. The inoculant was demonstrated to survive on inoculated seedlings 4 years after outplanting (56 months after inoculation), although S. collinitus was not fruiting. The designed markers set allows reliable and inexpensive monitoring of inoculated seedlings and suggests that S. collinitus is suitable for inoculation of Mediterranean Pinus . These data are discussed in the framework of suilloid population ecology.