Open AccessDepth related diversity of methanogen Archaea in Finnish oligotrophic fen
Author(s) -
Galand Pierre E.,
Saarnio Sanna,
Fritze Hannu,
Yrjälä Kim
Publication year - 2002
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.2002.tb01033.x
Subject(s) - biology , methanogen , archaea , methanomicrobiales , restriction fragment length polymorphism , terminal restriction fragment length polymorphism , genetics , methanosarcina , gene , bacteria , polymerase chain reaction
The annual rate of CH 4 release and potential CH 4 production has recently been studied in the Salmisuo fen in eastern Finland but the microbiota responsible for the CH 4 production has not been examined. The diversity of the methane producing Archaea was analysed, at different depths, in the most representative microsite ( Eriophorum lawn) of the fen. Methanogen populations were studied using primers amplifying a region of the methyl‐coenzyme M reductase gene. PCR products were analysed by denaturing gradient gel electrophoresis and restriction fragment length polymorphism (RFLP) analysis of clone libraries. A representative of each RFLP group was sequenced. The study revealed a change of the methanogen populations with depth. Sequences from the upper layers of the fen grouped in a novel ‘Fen cluster’ and were related to Methanomicrobiales. Sequences retrieved from the deeper layers of the fen were related to Methanosarcinales via the Rice Cluster‐I.