Open AccessDiversity of 3‐chloroaniline and 3,4‐dichloroaniline degrading bacteria isolated from three different soils and involvement of their plasmids in chloroaniline degradation
Author(s) -
Dejonghe Winnie,
Goris Johan,
Dierickx Ann,
Dobbeleer Veerle,
Crul Katrien,
Vos Paul,
Verstraete Willy,
Top Eva M.
Publication year - 2002
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.2002.tb01021.x
Subject(s) - biology , plasmid , bacteria , proteobacteria , microbiology and biotechnology , genetics , gene , 16s ribosomal rna
Attempts were made to isolate 3‐chloroaniline (3‐CA) and 3,4‐dichloroaniline (3,4‐DCA) degrading bacteria from the A‐ and B‐horizon of three different soils. A variety of 3‐CA degrading bacteria was obtained from all soils, whereas 3,4‐DCA degrading strains were only isolated from one soil. Amongst the 3‐CA and 3,4‐DCA degraders, two belong to the γ‐ Proteobacteria and seven to the β‐ Proteobacteria . Of the latter group, five are members of the family of the Comamonadaceae . Interestingly, all isolates contained an IncP‐1β plasmid. These plasmids could be divided into four major groups based on restriction digest patterns. While all plasmids that were detected in the isolates, except one, encode total degradation of 3‐CA, no indigenous plasmid that codes for total degradation of 3,4‐DCA was found. This is the first study that reports the presence of diverse transferable plasmids that encode mineralisation of 3‐CA in different 3‐CA degrading species.