Open AccessDiversity and linkage of replication and mobilisation genes in Bacillus rolling circle‐replicating plasmids from diverse geographical origins
Author(s) -
Mason Vince P.,
Syrett Natalie,
Hassanali Tasneem,
Osborn A.Mark
Publication year - 2002
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.2002.tb01013.x
Subject(s) - plasmid , biology , genetics , bacillus licheniformis , gene , bacillus subtilis , rolling circle replication , linkage (software) , bacteria , dna replication
The prevalence, diversity and linkage of pTA‐related plasmid rep and mob genes in 18 plasmids from three closely related species of Bacillus from diverse geographical locations have been investigated using PCR. pTA‐related rep and mob sequences were both amplified from 13 of the 18 plasmids. For one plasmid, pBM9, only a pTA‐related mob gene was amplified, whilst only a pTA‐related rep gene was amplified from the Bacillus licheniformis plasmid pBL2. No products were amplified for either gene from two other B. licheniformis plasmids or from a larger 16‐kb Bacillus subtilis plasmid, pBS6. Whilst simple gene detection suggests close linkage of both pTA‐related rep and mob genes, on most of these plasmids, sequence analysis of amplified genes revealed more complex linkage relationships varying with geographical origin, plasmid size and bacterial host.