Open AccessDistribution and diversity of thermophilic sulfate‐reducing bacteria within a Cu‐Pb‐Zn mine (Toyoha, Japan)
Author(s) -
Nakagawa Tatsunori,
Hanada Satoshi,
Maruyama Akihiko,
Marumo Katsumi,
Urabe Tetsuro,
Fukui Manabu
Publication year - 2002
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.2002.tb00981.x
Subject(s) - temperature gradient gel electrophoresis , thermophile , biology , sulfate reducing bacteria , 16s ribosomal rna , library , bacteria , sulfur , sulfate , microbiology and biotechnology , chemistry , genetics , organic chemistry
The distribution and diversity of thermophilic sulfate‐reducing bacteria at the Cu‐Pb‐Zn Toyoha underground mine, Japan, were investigated using denaturing gradient gel electrophoresis analysis based on the 16S rRNA gene, and sequence analysis of the dissimilatory sulfite reductase gene. Hydrothermal waters from different boreholes penetrating the Cu‐Pb‐Zn sulfide veins were collected and concentrated with a sterile filter (pore size: 0.2 μm) at sites A (64°C), B (71°C), and C (48°C). Microbial mats developed at sites A (53°C), B (66°C), and D (73°C) were harvested. The denaturing gel electrophoresis analysis showed 17 bacterial and three archaeal bands including two of spore‐forming, Gram‐positive sulfate‐reducing bacteria, Desulfotomaculum ‐like 16S rDNA sequences from site B. The phylogenetic analysis of 16 clone families of dissimilatory sulfite reductase genes indicated that they are Desulfotomaculum ‐, Thermodesulforhabdus ‐like sequences, and unresolved sequences. We obtained evidence of the diversity and distribution of microbes related to thermophilic sulfate‐reducing bacteria within effluent‐hydrothermal groundwater and microbial mats in the thermophilic subsurface environment of the Toyoha Mine.