Open AccessDevelopment of a conditional lethal system for a Streptomyces lividans strain and its use to investigate conjugative transfer in soil
Author(s) -
ClercBardin Sylvie,
Karray Fatma,
Frostegard Asa,
Pernodet JeanLuc,
Simonet Pascal
Publication year - 2001
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.2001.tb00889.x
Subject(s) - biology , strain (injury) , operon , bacteria , glycerol , microbiology and biotechnology , actinomycetales , genetics , streptomyces , mutant , biochemistry , gene , anatomy
In Streptomyces lividans , a conditional lethal mechanism was obtained by inactivation of gylB in the glycerol operon. The resulting strain was efficiently counter‐selected on glycerol‐containing medium but the mutation had no effect on the capacity of the strain to be maintained in non‐sterile soil samples and to transfer derivatives of the genetic element pSAM2. However, despite the efficiency of the donor counter‐selection system, the transfer of a pSAM2 derivative from S. lividans to indigenous soil bacteria was not detected.