Open AccessCosmopolitan distribution of phlD ‐containing dicotyledonous crop‐associated biocontrol pseudomonads of worldwide origin
Author(s) -
Wang Chunxia,
Ramette Alban,
Punjasamarnwong Patchara,
Zala Marcello,
Natsch Andreas,
MoënneLoccoz Yvan,
Défago Geneviève
Publication year - 2001
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.2001.tb00858.x
Subject(s) - biology , pseudomonas , botany , pseudomonadaceae , pseudomonas fluorescens , microbiology and biotechnology , genetics , bacteria
In biocontrol fluorescent pseudomonads, phlD encodes a polyketide synthase required for the synthesis of the antifungal compound 2,4‐diacetylphloroglucinol (Phl). Here, PCR‐restriction fragment length polymorphism analysis was used to compare phlD alleles in 77 dicot‐associated pseudomonads originating from various countries worldwide and 10 counterparts from a monocotyledonous host (wheat). The 16 restriction patterns obtained were mostly unrelated to geographic location or dicot host. Cluster analysis distinguished eight phlD clusters at a similarity level of 0.63. One cluster grouped 18 pseudomonads that produced also the antifungal polyketide pyoluteorin but could not assimilate D ‐galactose, D ‐galactonate lactone, D ‐sorbitol, L ‐arabinose, D ‐saccharate or D ‐xylose. These 18 pseudomonads, along with the eight pseudomonads from a second phlD cluster, were the only isolates that failed to deaminase 1‐aminocyclopropane‐1‐carboxylate (ACC), a rare root growth promotion trait. Overall, assessment of phlD polymorphism, ACC deaminase activity and catabolic profiles pointed to a cosmopolitan distribution of Phl‐producing biocontrol fluorescent pseudomonads of worldwide origin associated with dicotyledonous crop plants.