Open AccessArchaeal communities in two disparate deteriorated ancient wall paintings: detection, identification and temporal monitoring by denaturing gradient gel electrophoresis
Author(s) -
Piñar Guadalupe,
SaizJimenez Cesareo,
SchabereiterGurtner Claudia,
BlancoVarela Maria Teresa,
Lubitz Werner,
Rölleke Sabine
Publication year - 2001
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.2001.tb00852.x
Subject(s) - temperature gradient gel electrophoresis , biology , archaea , 16s ribosomal rna , halophile , clone (java method) , library , genetics , dna , bacteria
In the present study we describe the detection of Archaea in two different deteriorated ancient wall paintings, located in Austria and Spain, under different humidity/salinity regimes and climates. Archaeal communities were analyzed by combination of denaturing gradient gel electrophoresis (DGGE) of PCR‐amplified DNA encoding 16S rRNA and the construction of clone libraries. DGGE analysis was used for temporal monitoring of the archaeal communities as well as for screening of the clone libraries. Selected clones were compared with band patterns obtained from the original samples and sequenced. Sequence analyses documented the existence of species belonging to well‐characterized halophilic and alkaliphilic Archaea .