Open AccessGeographic distribution of Desulfitobacterium frappieri PCP‐1 and Desulfitobacterium spp. in soils from the province of Quebec, Canada
Author(s) -
Lanthier Martin,
Villemur Richard,
Lépine François,
Bisaillon JeanGuy,
Beaudet Réjean
Publication year - 2001
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.2001.tb00839.x
Subject(s) - biology , soil water , polymerase chain reaction , soil microbiology , ribosomal rna , 16s ribosomal rna , bacteria , ecology , gene , genetics
The presence of indigenous Desulfitobacterium species in 44 soil samples taken from various sites in the southern part of the province of Quebec (Canada) and four from locations outside Quebec was investigated. Twenty‐four of these soils were sampled from contaminated industrial sites. Indigenous Desulfitobacterium bacteria from soil samples were enriched by cultivation in anaerobic soil slurry culture. Total DNA was then extracted from these slurries and polymerase chain reaction (PCR) amplifications were performed with primers targeting 16S ribosomal RNA gene sequences of Desulfitobacterium spp. and of Desulfitobacterium frappieri PCP‐1. A positive PCR signal was obtained in 31 soil slurry cultures. Resolution of single‐strand DNAs of some of the PCR products by a single‐strand conformational polymorphism protocol suggests that more than one species of Desulfitobacterium were present in the corresponding slurry cultures. These results suggest that Desulfitobacterium are ubiquitous in soils in the province of Quebec, especially in soils from the St. Lawrence valley and the southern part of the province.