Open AccessEvaluation of the denitrifying microbiota of anoxic reactors
Author(s) -
Etchebehere Claudia,
Errazquin Inés,
Barrandeguy Elena,
Dabert Patrick,
Moletta René,
Muxı́ Lucı́a
Publication year - 2001
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.2001.tb00811.x
Subject(s) - denitrifying bacteria , anoxic waters , biology , denitrification , microbiology and biotechnology , microorganism , environmental chemistry , bacteria , ecology , nitrogen , chemistry , genetics , organic chemistry
Removal of inorganic nitrogen compounds from wastewaters can be accomplished by a combination of the biological processes of nitrification and denitrification. The information on the microbiota present in denitrifying reactors is still scarce. In the present work the evaluation of the denitrifying microbiota of different reactor sludges was performed by specific activity measurements and MPN count of denitrifiers. We also present the isolation and physiological and phylogenetic characterisation of denitrifying bacteria from the anoxic reactor of a combined system treating landfill leachate. Specific denitrifying activity measurements were faster to perform and more reliable than MPN enumerations. 16S rDNA characterisation of the isolates showed that they belonged to the genera Thauera , Acidovorax and Alcaligenes and were closely related to microorganisms retrieved from ecosystems rich in recalcitrant compounds. Two of the isolates could grow on aromatic compounds as sole carbon source.