Open AccessResponse of a Pseudomonas aeruginosa biofilm community to DNA‐damaging chemical agents
Author(s) -
Elasri Mohamed O,
Reid Tricia,
Hutchens Steven,
Miller Robert V
Publication year - 2000
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.2000.tb00722.x
Subject(s) - biology , biofilm , pseudomonas aeruginosa , microbiology and biotechnology , operon , plasmid , bacteria , vibrio cholerae , pseudomonas , vibrio , dna , gene , escherichia coli , genetics
Pseudomonas aeruginosa strain RM4440 carries a plasmid‐borne fusion of the P. aeruginosa recA gene promoter to a promoterless lux operon from Vibrio fisheri . We tested the response of RM4440 in a biofilm to exposure to a 1‐h pulse of each of 17 chemicals known to be toxic to bacteria and other organisms. The induction of light produced from the recA–lux fusion present in RM4440 proved to be sensitive and specific for DNA‐damaging chemicals when included in a biofilm environment. This study demonstrates the potential usefulness of this construct for in situ investigation of bacterial communities.