Open AccessMonitoring impact of in situ biostimulation treatment on groundwater bacterial community by DGGE
Author(s) -
Iwamoto Tomotada,
Tani Katsuji,
Nakamura Kanji,
Suzuki Yoshihiko,
Kitagawa Masayoshi,
Eguchi Masahiro,
Nasu Masao
Publication year - 2000
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.2000.tb00707.x
Subject(s) - temperature gradient gel electrophoresis , methane monooxygenase , biostimulation , biology , methanotroph , 16s ribosomal rna , microbiology and biotechnology , microbial population biology , gel electrophoresis , bacteria , food science , methane , biochemistry , ecology , bioremediation , genetics , anaerobic oxidation of methane
Changes in bacterial diversity during the field experiment on biostimulation were monitored by denaturing gradient gel electrophoresis (DGGE) analysis of PCR‐amplified 16S rDNA fragments. The results revealed that the bacterial community was disturbed after the start of treatment, continued to change for 45 days or 60 days and then formed a relatively stable community different from the original community structure. DGGE analysis of soluble methane monooxygenase (sMMO) hydroxylase gene fragments, mmoX , was performed to monitor the shifts in the numerically dominant sMMO‐containing methanotrophs during the field experiment. Sequence analysis on the mmoX gene fragments from the DGGE bands implied that the biostimulation treatment caused a shift of potential dominant sMMO‐containing methanotrophs from type I methanotrophs to type II methanotrophs.