Open AccessBacterial survival and mineralization of p ‐nitrophenol in soil by green fluorescent protein‐marked Moraxella sp. G21 encapsulated cells
Author(s) -
Errampalli Deena,
Tresse Odile,
Lee Hung,
Trevors Jack T.
Publication year - 1999
Publication title -
fems microbiology ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.377
H-Index - 155
eISSN - 1574-6941
pISSN - 0168-6496
DOI - 10.1111/j.1574-6941.1999.tb00651.x
Subject(s) - biology , mineralization (soil science) , microbiology and biotechnology , moraxella , green fluorescent protein , bioremediation , bacteria , biochemistry , gene , soil water , ecology , genetics
Moraxella sp. G21 cells marked with the green fluorescent protein ( gfp ) survived in κ‐carrageenan beads and as free cells for a month after inoculation into autoclaved soil and non‐sterile soil contaminated with p ‐nitrophenol (PNP). Similar [U‐ 14 C]PNP mineralization values were produced by encapsulated Moraxella sp. G21 cells and as free cells (53 and 60% mineralization). There was no significant difference between cell survival and [U‐ 14 C]PNP mineralization activity in soil by the rifampicin‐resistant Moraxella sp. mental strain and Moraxella sp. G21. The ability of encapsulated Moraxella sp. G21 cells to survive, retain their green fluorescence and mineralize [U‐ 14 C]PNP suggests that the GFP‐marked strain encapsulated in κ‐carrageenan may be useful for bioremediation of toxic chemicals in soil.